Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis.
نویسندگان
چکیده
The global yield of bananas-one of the most important food crops-is severely hampered by parasites, such as nematodes, which cause yield losses up to 75%. Plant-nematode interactions of two banana cultivars differing in susceptibility to Radopholus similis were investigated by combining the conventional and spatially resolved analytical techniques (1)H NMR spectroscopy, matrix-free UV-laser desorption/ionization mass spectrometric imaging, and Raman microspectroscopy. This innovative combination of analytical techniques was applied to isolate, identify, and locate the banana-specific type of phytoalexins, phenylphenalenones, in the R. similis-caused lesions of the plants. The striking antinematode activity of the phenylphenalenone anigorufone, its ingestion by the nematode, and its subsequent localization in lipid droplets within the nematode is reported. The importance of varying local concentrations of these specialized metabolites in infected plant tissues, their involvement in the plant's defense system, and derived strategies for improving banana resistance are highlighted.
منابع مشابه
Aggressiveness and Damage Potential of Central American and Caribbean Populations of Radopholus spp. in Banana.
Monoxenic cultures of burrowing nematode populations extracted from banana roots from Belize, Guatemala, Honduras, and Costa Rica were established on carrot discs. Cultures of Radopholus spp. were also obtained from Florida, Puerto Rico, Dominican Republic, and Ivory Coast. The aggressiveness (defined as reproductive fitness and root necrosis) of these populations was evaluated by inoculating b...
متن کاملWorldwide dissemination of Radopholus similis and its importance in crop production.
The burrowing nematode, Radopholus similis, attacks agronomic and horticultural crops and many weeds, and is reported to reproduce on more than 250 plant species. Two races of R. similis are recognized. Although one race attacks citrus and the other race does not, they are morphologically similar. At present, the citrus race is found attacking citrus only in Florida, U.S.A., but it is known to ...
متن کاملCharacterization of a New Burrowing Nematode Population, Radopholus citrophilus, from Hawaii.
Karyotype, host preference, isozyzme patterns, morphometrics, and mating behavior of two burrowing nematode populations from Hawaii, one infecting Anthurium sp. and the second infecting Musa sp., were compared with Radopholus similis and R. citrophilus populations from Florida. The population from Anthurium sp. had five chromosomes (n = 5), and that from Musa sp. had four (n = 4). Neither of th...
متن کاملAseptic Culture Systems of Radopholus similis for In Vitro Assays on Musa spp. and Arabidopsis thaliana.
Radopholus similis is one of the most damaging nematodes in bananas. Chemical control is currently the most-used method, but nematode control through genetic improvement is widely encouraged. The objective of this study was to establish an aseptic culture system for R. similis and determine whether R. similis can infect and reproduce on in vitro banana plantlets and in vitro Arabidopsis thalian...
متن کاملPotential physical and chemical barriers to infection by the burrowing nematode Radopholus similis in roots of susceptible and resistant banana ( Musa spp.)
a Laboratory of Tropical Crop Improvement, Division of Crop Biotechnics, Catholic University of Leuven (K.U. Leuven), Kasteelpark Arenberg 13, 3001 Leuven; b Unité de Chimie Analytique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés 2, 5030 Gembloux; and c Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passa...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 111 1 شماره
صفحات -
تاریخ انتشار 2014